Scientist I/II, Preclinical Research

  We are seeking a detail-oriented and experienced Scientist to join our Preclinical Development team to support the validation of gene editing therapies and determine cellular and molecular activity. Reporting to the Principal Scientist, this position will support the development of a gene-editing project working on Hemophilia. You will use your experience with single-plex and multiplex digital droplet PCR, culturing primary cells, and purifying/isolating gDNA and mRNA from cells and animal tissue to contribute to gene-editing advancements focused on hemophilia.  The ideal candidate will have strong skills in cell and molecular biology and will be motivated to collaborate with teams to optimize assays to deliver groundbreaking therapies. This role will be based in Emeryville, California. How you'll contribute As a member of a dynamic and scientifically rigorous team, you will contribute to the development of meaningful gene-editing advancements focused on Hemophilia Harvest cells and tissue in preparation for various assays such as PCR for next-generation sequencing   Design, execute and analyze cellular and molecular assays to generate data to determine on-target and off-target activity Analyze tissues through molecular experiments and manage data appropriately Observe target penetration of therapy through methods such as FACS, ELISA, and next-gen sequencing technologies Transfect cells and prepare and optimize for analysis; work with computation teams to drive analysis and interpretation of activity Perform molecular/cellular cloning Collaborate with computational scientists and engineers to analyze data and present findings to the larger team Purify/Isolate gDNA and mRNA from cells and animal tissue  What you bring Ph.D. in biology with at least two years of industry experience or BS/MS with at least three years of highly relevant industry experience in a closely related discipline Advanced proficiency with single-plex and multiplex digital droplet PCR Expertise in culturing primary cells, purifying/isolating gDNA and mRNA from cells and animal tissue, and transfections of primary cells  Demonstrated experience working with primary cell systems and performing downstream molecular assays A strong track record of working with large data and applying algorithms Experience with ELISA and/or electrochemiluminescence (ECL) based protein detection methods (e.g., Meso Scale Discovery) Understanding or experience with Next-Generation Sequencing workflow  Experience in molecular cloning, vector building, and CRISPR-based gene editing systems and knock-in strategies Understanding of lipid nanoparticle (LNP) technology and mRNA-based therapeutics Effective oral and written communication skills, particularly when collaborating with other teams with limited knowledge of your area of expertise Extremely organized and able to efficiently manage your time and workflow, including skillfully prioritizing both long-term projects and day-to-day responsibilities Ability to quickly learn and adapt to new software tools (Google Suite, Slack, Geneious, CRISPR and Off-target algorithms, NGS) A highly collaborative and team-oriented mindset, who thrives in a fast-moving, results-oriented startup environment  California Pay Transparency Disclosure: This position is classified as exempt under the Federal Labor Standards Act.  This position's anticipated base salary range will be $115,000 to $145,000.  You will also be eligible to receive equity, bonuses, and a full range of medical, financial, and other benefits depending on the position offered. Please note that individual total compensation for this position will be determined at the Company’s sole discretion and may vary based on location, skill level, years and depth of relevant experience, and education.    #LI-NG1 About Metagenomi Metagenomi is a gene editing company committed to developing potentially curative therapeutics by leveraging a proprietary toolbox of next-generation gene editing systems to accurately edit DNA where current technologies cannot. Our metagenomics-powered discovery platform and analytical expertise reveal novel cellular machinery sourced from otherwise unknown organisms. We adapt and forge these naturally evolved systems into powerful gene editing systems that are ultra-small, extremely efficient, highly specific and have a decreased risk of immune response. These systems fuel our pipeline of novel medicines and can be leveraged by partners. Our goal is to revolutionize gene editing for the benefit of patients around the world. For more information, please visit https://metagenomi.co/.   What makes Metagenomi a unique place to work? We are passionate about developing technologies derived from microbial-rich ecosystems that have the power to enable therapeutic development and the potential to cure devastating disease. We have cultivated an amazing culture that values teamwork, curiosity, scientific rigor, and fun. We have a strong commitment to work/life balance, host a weekly employee happy hour and events, provide catered lunches and endless snacks, and more!  We offer competitive compensation including a market-benchmarked salary, annual target bonus, equity in an early-stage startup, and a comprehensive benefits plan, including 401(k) matching, a retirement safe harbor plan, and other perks.   At Metagenomi, we know that our people drive our success. We are an equal-opportunity employer and believe in and value diversity and inclusion. Metagenomi provides an open, collegial, and friendly work environment where we empower people and provide them with opportunities to develop their long-term careers. Metagenomi does not discriminate on the basis of race, color, religion, sexual orientation, gender, age, physical or mental disability, or any other status protected under federal, state, or local law. Please note, all candidates must be authorized to work in the United States.   Applicants/Employees have rights under various State and Federal Employment Laws, including without limitation:   Family & Medical Leave Act    Equal Employment Opportunity Commission   Employee Polygraph Protection Act    California Family Rights Act    California Fair Employment and Housing Act   Notice to agencies: Our in-house Talent Acquisition Team manages all employment opportunities at Metagenomi. Agencies and independent recruiters must be approved as a vendor by Metagenomi’s Talent Acquisition team. To protect the interests of all parties, Metagenomi will not accept unsolicited resumes, profiles, or biographies. Any unsolicited resumes sent to Metagenomi will be considered referrals and the property of Metagenomi.